Expression Profiling of Lymphocyte Plasma Membrane Proteins
نویسندگان
چکیده
منابع مشابه
Phosphorylated lymphocyte plasma-membrane proteins.
Lymphocytes were labelled by incubation with [32P]Pi and their plasma membranes isolated. Analysis by one-dimensional and two-dimensional gel electrophoresis revealed a small number of strongly phosphorylated polypeptides. Two of these were especially prominent; they had molecular weights of about 52000 and 90000, were acidic and were apparently not glycosylated. Similar patterns were obtained ...
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Blood-brain barrier (BBB) membrane proteins play crucial roles in the proper functioning of the BBB as well as in disease progression. Previously, we developed a novel approach for identifying membrane proteins expressed at the BBB, which we referred to as multiplex expression cloning. In this study, the proteome coverage of the multiplex expression cloning approach was expanded to allow the id...
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Infection of the mouse peritoneal cavity by bacillus Calmette-Gu6rin (BCG) 1 markedly alters the surface properties of macrophages (mob), compard with cells obtained from uninfected control animals or after injection of thioglycollate broth. BCG-activated peritoneal mob (BCG-PM) express enhanced Ia antigens (Ag), but reduced receptors for mannose-terminated glycoconjugates (MFR), Fc receptors, ...
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Between 30 and 50% of pig lymphocyte plasma membrane vesicles were not bound by concanavalin A (Con A)-Sepharose. Various results suggest that the Con A-unretarded fraction represents "inside-out" membrane vesicles. First, an alternative cell surface ligand, anti-lymphocytic serum, gave a similar fractionation to Con A. Second, lack of binding by Con A was not due to lack of carbohydrate or to ...
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serum stained rat liver and kidney glomeruli only. It remains to be determined whether the anti-bodies against the 65000and/or 43000-moI.wt. membrane components are responsible for these staining patterns and whether the above difference between the anti(inside-out membrane) and anti-(actin) sera reflect differences between the antigenicities of non-muscle and muscle actins. The present results...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2004
ISSN: 1535-9476
DOI: 10.1074/mcp.m300064-mcp200